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Virucidal Research Test

Test Method: * U.S. E.P.A. Pesticide Assessment Guidelines, Subdivision G: Product Performance, Section 91-2 (f), and Section 91-30, (d), (e), November 1982. † Protocols for Testing the Efficacy of Disinfectants against Hepatitis B Virus (HBV) (EPA, Federal Register, Vol. 65, No. 166, 8/25/2000, p. 51828). ‡ Protocol for Testing Disinfectants against Hepatitis C Virus using Bovine Viral Diarrhea Virus as approved by the U.S. EPA on August 15, 2002.

Test Conditions: 10 minute contact time, 5% organic soil load, sterile glass petri dishes, 400 ppm hard water, 21-24°C exposure temperature, 4 oz/gal dilution

Virucidal Data Table


Test Organism Sample Titer Reduction
Avian Influenza A Virus (H3N2) (Avian Ressortant)
(ATCC VR-2072)


≥4.25 log10


≥4.25 log10

*Avian Influenza Virus, Type A (Turkey/WIS/66) (H9N2) A ≥4.0 log10
B ≥4.0 log10
*Bovine Rhinotracheitis, strain LA (ATCC VR-188) A ≥5.0 log10
B ≥5.0 log10
‡Bovine Viral Diarrhea Virus (BVDV) A 5.9 log10
B 5.9 log10
*Canine Distemper Virus, strain Lederle (ATCC VR-128) A ≥6.25 log10
B ≥6.25 log10
*Feline Picornavirus, strain FRV (ATCC VR-649) A ≥4.25 log10
B ≥4.25 log10
†Hepatitis B Virus (HBV) (Duck Hepatitis B Virus-DHBV) A 4.5 log10
B 4.7 log10
‡Hepatitis C Virus (HCV) (Bovine Viral Diarrhea Virus-BVDV) A 5.9 log10
B 5.9 log10
*Herpes Simplex Type 1 (ATCC VR-260) A ≥5.0 log10
B ≥5.0 log10
*Herpes Simplex Type 2 (ATCC VR-734) A ≥6.0 log10
B ≥6.0 log10
*Human Coronavirus (ATTC VR-740, strain 229E) A ≥3.0 log10
B ≥3.0 log10

*Human Immunodeficiency Virus, HTLV-IIIRF, strain of HIV-1

(associated with AIDS)

A ≥3.5 log10
B ≥3.5 log10
*Human Immunodeficiency Virus type 2 (HIV-2), strain
A ≥3.25 log10
B ≥3.25 log10
*Influenza A2, strain Hong Kong (ATCC VR-544) A ≥4.25 log10
B ≥4.25 log10
*Pandemic 2009 H1N1 Influenza A Virus (Refer to Note on next page.)
*Paramyxovirus (Mumps) (ATCC VR-1438) A ≥3.0 log10
B ≥3.0 log10

*Porcine Respiratory & Reproductive Syndrome Virus

(PRRSV), strain NVSL

A ≥5.0 log10
B ≥5.0 log10
*Pseudorabies, strain Aujeszky (ATCC VR-135) A ≥5.25 log10
B ≥5.25 log10
*Rabies Virus (attenuated CDC ERA strain) A 3.0 log10
B 3.0 log10
*Rotavirus, strain SA-11 (ATCC VR-899) A 4.5 log10
B 4.5 log10
*SARS Associated Coronavirus (ZeptoMetrix) A 3.03 log10
B 3.03 log10
*Vaccinia, strain WR (ATCC VR-119) A ≥5.5 log10
B ≥5.5 log10

Virucidal Data Conclusions

Conclusions: Under the conditions of this investigation, Calla® 1452 demonstrated virucidal activity against Avian Influenza A Virus (H3N2), Avian Influenza Virus, Type A (H9N2), Bovine Rhinotracheitis, Bovine Viral Diarrhea Virus (BVDV), Canine Distemper Virus, Feline Picornavirus, Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Herpes Simplex Type 1, Herpes Simplex Type 2, Human Coronavirus, Human Immunodeficiency Virus (HIV-1), Human Immunodeficiency Virus Type 2 (HIV-2), Influenza A2, Pandemic 2009 H1N1 Influenza A Virus, Paramyxovirus (Mumps), Porcine Respiratory & Reproductive Syndrome Virus (PRRSV), Pseudorabies, Rabies Virus, Rotavirus, SARS Associated Coronavirus and Vaccinia according to criteria established by the U.S. Environmental Protection Agency for registration and labeling of a disinfectant product as a virucide.

Note: Per the EPA guidance document dated October 21, 2009, disinfectant products that bear label claims against human, avian, or swine influenza A virus, and have submitted and received approval of efficacy data to support these label claims, may include a label claim against the Pandemic 2009 H1N1 Influenza A Virus.

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