Test Method: * U.S. E.P.A. Pesticide Assessment Guidelines, Subdivision G: Product Performance, Section 91-2 (f), and Section 91-30, (d), (e), November 1982.
† Protocols for Testing the Efficacy of Disinfectants against Hepatitis B Virus (HBV) (EPA, Federal Register, Vol. 65, No. 166, 8/25/2000, p. 51828).
‡ Protocol for Testing Disinfectants against Hepatitis C Virus using Bovine Viral Diarrhea Virus as approved by the U.S. EPA on August 15, 2002.
Test Conditions: 10 minute contact time, 5% organic soil load, sterile glass petri dishes, 400 ppm hard water, 21-24°C (70°F-75°F) exposure temperature, and 4 oz per gallon dilution.
Results:
Test Organism | Sample | Titer Reduction | Contact Time |
*SARS-Related Coronavirus 2 [SARS-CoV-2] [USA-WA1/2020] [BEI NR-52281] | – | – | 1 Minute |
*Avian Influenza A Virus (H3N2) (Avian Ressortant) (ATCC VR-2072) |
A |
≥4.25 log10 |
10 minutes |
*Avian Influenza Virus, Type A (Turkey/WIS/66) (H9N2) |
A |
≥4.0 log10 |
10 minutes |
*Bovine Rhinotracheitis, strain LA (ATCC VR-188) |
A |
≥5.0 log10 |
10 minutes |
‡Bovine Viral Diarrhea Virus (BVDV) |
A |
5.9 log10 |
10 minutes |
*Canine Distemper Virus, strain Lederle (ATCC VR-128) |
A |
≥6.25 log10 |
10 minutes |
Feline Leukemia (VR-717) | – | – | 10 minutes |
*Feline Picornavirus, strain FRV (ATCC VR-649) |
A |
≥4.25 log10 |
10 minutes |
†Hepatitis B Virus (HBV) (Duck Hepatitis B Virus-DHBV) |
A |
4.5 log10 |
10 minutes |
‡Hepatitis C Virus (HCV) (Bovine Viral Diarrhea Virus-BVDV) |
A |
5.9 log10 |
10 minutes |
*Herpes Simplex Type 1 (ATCC VR-260) |
A |
≥5.0 log10 |
10 minutes |
*Herpes Simplex Type 2 (ATCC VR-734) |
A |
≥6.0 log10 |
10 minutes |
*Human Coronavirus (ATTC VR-740, strain 229E) |
A |
≥3.0 log10 |
10 minutes |
*Human Immunodeficiency Virus, HTLV-IIIRF, strain of HIV-1 (associated with AIDS) |
A |
≥3.5 log10 |
10 minutes |
*Human Immunodeficiency Virus type 2 (HIV-2), strain CBL-20 |
A |
≥3.25 log10 |
10 minutes |
*Influenza A2, strain Hong Kong (ATCC VR-544) |
A |
≥4.25 log10 |
10 minutes |
*Paramyxovirus (Mumps) (ATCC VR-1438) |
A |
≥3.0 log10 |
10 minutes |
*Porcine Respiratory & Reproductive Syndrome Virus (PRRSV), strain NVSL |
A |
≥5.0 log10 |
10 minutes |
*Pseudorabies, strain Aujeszky (ATCC VR-135) |
A |
≥5.25 log10 |
10 minutes |
*Rabies Virus (attenuated CDC ERA strain) |
A |
3.0 log10 |
10 minutes |
*Rotavirus, strain SA-11 (ATCC VR-899) |
A |
4.5 log10 |
10 minutes |
*SARS Associated Coronavirus (ZeptoMetrix) |
A |
3.03 log10 |
10 minutes |
*Vaccinia, strain WR (ATCC VR-119) |
A |
≥5.5 log10 |
10 minutes |
Conclusions: Under the conditions of this investigation, Calla® 1452 RTU demonstrated virucidal activity against SARS-Related Coronavirus 2 (SARS-CoV2), Avian Influenza A Virus (H3N2), Avian Influenza Virus, Type A (H9N2), Bovine Rhinotracheitis, Bovine Viral Diarrhea Virus (BVDV), Canine Distemper Virus, Feline Leukemia (VR-717), Feline Picornavirus, Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Herpes Simplex Type 1, Herpes Simplex Type 2, Human Coronavirus, Human Immunodeficiency Virus (HIV-1), Human Immunodeficiency Virus Type 2 (HIV-2), Influenza A2, Paramyxovirus (Mumps), Porcine Respiratory & Reproductive Syndrome Virus (PRRSV), Pseudorabies, Rabies Virus, Rotavirus, SARS Associated Coronavirus, and Vaccinia, according to criteria established by the U.S. Environmental Protection Agency for registration and labeling of a disinfectant product as a virucide.