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Calla® 1452 RTU Disinfection Data

Test Method: AOAC Use Dilution

Test Conditions: 5% organic soil load, 10 minute contact time, stainless steel carrier substrates, 400 ppm hard water, 20°C exposure temperature, and 4 oz/gal dilution.

Results:

 

No. of Carriers

Test Organism

Sample Exposed

Positive

Contact Time

Ampicillin resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Bactrim resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Bordetella bronchiseptica (ATCC 31437)

A
B

10
10

0
0

10 minutes
Cefazolin resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Ceftazdime resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Ceftriaxone resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Ciprofloxacin resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Community Associated Methicillin Resistant Staphylococcus aureus (CA-MRSA) (NRS 123, Genotype USA400)

A
B
C

10
10
10

0
0
0

10 minutes
Community Associated Methicillin Resistant Staphylococcus aureus (CA-MRSA) (NRS 384, Genotype USA300)

A
B
C

10
10
10

0
0
0

10 minutes
Corynebacterium ammoniagenes (ATCC 6871)

A
B

10
10

0
0

10 minutes
Enterobacter aerogenes (ATCC 13048)

A
B

10
10

0
0

10 minutes
Enterobacter cloacae (ATCC 23355)

A
B

10
10

0
0

10 minutes
Enterobacter cloacae (clinical isolate)

A
B

10
10

0
0

10 minutes
Enterococcus faecalis (ATCC 19433)

A
B

10
10

0
0

10 minutes
Enterococcus faecalis (clinical isolate)

A
B

10
10

0
0

10 minutes
Escherichia coli (ATCC 11229)

A
B

10
10

0
0

10 minutes
Escherichia coli (clinical isolate)

A
B

10
10

0
0

10 minutes
Escherichia coli O111:H8 (BAA-184)

10 minutes
Fusobacterium necrophorum (ATCC 27852)

A
B

10
10

0
0

10 minutes
Gentamicin resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Klebsiella pneumoniae subsp. pneumoniae (ATCC 13883)

A
B

10
10

0
0

10 minutes
Lactobacillus casei subsp. rhamnosus (ATCC 7469)

A
B

10
10

0
0

10 minutes
Levofloxacin resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Listeria monocytogenes (ATCC 35152)

A
B

10
10

0
0

10 minutes
Methicillin Resistant Staphylococcus aureus (MRSA) (ATCC 33592)

A
B

10
10

0
0

10 minutes
Pasteurella multocida (ATCC 7707)

A
B

10
10

0
0

10 minutes
Proteus mirabilis (ATCC 9921)

A
B

10
10

0
0

10 minutes
Proteus mirabilis (ATCC 25933)

A
B

10
10

0
0

10 minutes
Proteus vulgaris (ATCC 13315)

A
B

10
10

0
0

10 minutes
Pseudomonas aeruginosa PRD-10 (ATCC 15442)

A
B
C

60
60
60

0
0
1

10 minutes
Salmonella enterica subsp. enterica serotype choleraesuis (ATCC 10708)

A
B
C

60
60
60

0
0
1

10 minutes
Salmonella enterica subsp. enterica serotype paratyphi B (ATCC 8759)

A
B

10
10

0
0

10 minutes
Salmonella enterica subsp. enterica serotype pullorum (ATCC 9120)

A
B

10
10

0
0

10 minutes
Salmonella enterica subsp. enterica serotype typhi (ATCC 6539)

A
B

10
10

0
0

10 minutes
Salmonella enterica subsp. enterica serotype typhimurium (ATCC 14028)

A
B

10
10

0
0

10 minutes
Salmonella enteritidis subsp. Enterica serotype enteritidis (ATCC 13076)

A
B

10
10

0
0

10 minutes
Serratia marcescens (ATCC 8100)

A
B

10
10

0
0

10 minutes
Shigella dysenteriae (ATCC 12180)

A
B

10
10

0
0

10 minutes
Shigella flexneri Type 2b (ATCC 12022)

A
B

10
10

0
0

10 minutes
Shigella sonnei (ATCC 25931)

A
B

10
10

0
0

10 minutes
Staphylococcus aureus (ATCC 6538)

A
B
C

60
60
60

1
1
1

10 minutes
Staphylococcus aureus subsp. aureus (ATCC 33592)

A
B

10
10

0
0

10 minutes
Staphylococcus aureus (clinical isolate)

A
B

10
10

0
0

10 minutes
Staphylococcus epidermidis (ATCC 29641)

A
B

10
10

0
0

10 minutes
Staphylococcus epidermidis (clinical isolate)

A
B

10
10

0
0

10 minutes
Streptococcus pyogenes Group A (ATCC 19615)

A
B

10
10

0
0

10 minutes
Streptococcus pyogenes (clinical-flesh eating strain, BIRD M3)

A
B

10
10

0
0

10 minutes
Tobramycin resistant Acinetobacter baumannii (clinical isolate-Fairfax Hospital CI 02001)

A
B

10
10

0
0

10 minutes
Vancomycin Resistant Enterococcus faecalis (VRE) (ATCC 51575)

A
B

10
10

0
0

10 minutes
Vancomycin Intermediate Resistant Staphylococcus aureus (VISA) (HIP-5836)

A
B

10
10

0
0

10 minutes
Xanthamonas maltophilia (clinical isolate)

A
B

10
10

0
0

10 minutes
Xanthomonas axonopodis pathovar citri @ a dilution ratio of 1:13.5 (2000 ppm active quaternary)

A
B

10
10

0
0

 

Conclusions: Under the conditions of this investigation, Calla® 1452 RTU demonstrated disinfectant activity against Staphylococcus aureus, Salmonella (choleraesuis) enterica, Pseudomonas aeruginosa PRD-10, Ampicillin resistant Acinetobacter baumannii, Bactrim resistant Acinetobacter baumannii, Bordetella bronchiseptica, Cefazolin resistant Acinetobacter baumannii, Ceftazdime resistant Acinetobacter baumannii, Ceftriaxone resistant Acinetobacter baumannii, Ciprofloxacin resistant Acinetobacter baumannii, Community Associated Methicillin Resistant Staphylococcus aureus (CAMRSA) (NRS 123, Genotype USA400), Community Associated Methicillin Resistant Staphylococcus aureus (CA-MRSA) (NRS384, Genotype USA300), Corynebacterium ammoniagenes, Enterobacter aerogenes, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli, Fusobacterium necrophorum, Gentamicin resistant Acinetobacter baumannii, Levofloxacin resistant Acinetobacter baumannii, Klebsiella pneumonia subsp. pneumoniae, Lactobacillus casei subsp. rhamnosus, Listeria monocytogenes, Methicillin Resistant Staphylococcus aureus (MRSA), Pasteurella multocida, Proteus mirabilis (ATCC 9921), Proteus mirabilis (ATCC 25933), Proteus vulgaris, Salmonella (paratyphi B) enterica, Salmonella (pullorum) enterica, Salmonella (typhi) enterica, Salmonella (typhimurium) enterica, Salmonella (enteritidis) enterica, Serratia marcescens, Shigella dysenteriae, Shigella flexneri Type 2b, Shigella sonnei, Staphylococcus aureus subsp. aureus, Staphylococcus epidermidis, Streptococcus pyogenes Group A, Streptococcus pyogenes (clinical flesh eating strain, BIRD M3), Tobramycin resistant Acinetobacter baumannii, Vancomycin Resistant Enterococcus faecalis (VRE), and Vancomycin Intermediate Resistant Staphylococcus aureus (VISA), according to criteria established by the U.S. Environmental Protection Agency for registration and labeling of a disinfectant product as a bactericide.

Calla® 1452 RTU also demonstrated disinfectant activity against the following antibiotic resistant clinical isolates: Enterobacter cloacae, Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Xanthamonas maltophilia. At a dilution ratio of 1:13.5 (2000 ppm active quaternary), Calla® 1452 RTU demonstrated disinfectant activity against Xanthomonas axonopodis pathovar citri (Citrus Canker Disease).