Calla® 1452 Virucidal Data

Test Method: * U.S. E.P.A. Pesticide Assessment Guidelines, Subdivision G: Product Performance, Section 91-2 (f), and Section 91-30, (d), (e), November 1982.

† Protocols for Testing the Efficacy of Disinfectants against Hepatitis B Virus (HBV) (EPA, Federal Register, Vol. 65, No. 166, 8/25/2000, p. 51828).

‡ Protocol for Testing Disinfectants against Hepatitis C Virus using Bovine Viral Diarrhea Virus as approved by the U.S. EPA on August 15, 2002.

Test Conditions: 10 minute contact time, 5% organic soil load, sterile glass petri dishes, 400 ppm hard water, 21-24°C (70°F-75°F) exposure temperature, and 4 oz per gallon dilution.

Results:

Test Organism	Sample	Titer Reduction	Contact Time
*SARS-Related Coronavirus 2 [SARS-CoV-2] [USA-WA1/2020] [BEI NR-52281]	A B	–	1 minute
*Avian Influenza A Virus (H3N2) (Avian Ressortant) (ATCC VR-2072)	A B	≥4.25 log₁₀ ≥4.25 log₁₀	10 minutes
*Avian Influenza Virus, Type A (Turkey/WIS/66) (H9N2)	A B	≥4.0 log₁₀ ≥4.0 log₁₀	10 minutes
*Bovine Rhinotracheitis, strain LA (ATCC VR-188)	A B	≥5.0 log₁₀ ≥5.0 log₁₀	10 minutes
‡Bovine Viral Diarrhea Virus (BVDV)	A B	5.9 log₁₀ 5.9 log₁₀	10 minutes
*Canine Distemper Virus, strain Lederle (ATCC VR-128)	A B	≥6.25 log₁₀ ≥6.25 log₁₀	10 minutes
Feline Leukemia (VR-717)	–	–	10 minutes
*Feline Picornavirus, strain FRV (ATCC VR-649)	A B	≥4.25 log₁₀ ≥4.25 log₁₀	10 minutes
†Hepatitis B Virus (HBV) (Duck Hepatitis B Virus-DHBV)	A B	4.5 log₁₀ 4.7 log₁₀	10 minutes
‡Hepatitis C Virus (HCV) (Bovine Viral Diarrhea Virus-BVDV)	A B	5.9 log₁₀ 5.9 log₁₀	10 minutes
*Herpes Simplex Type 1 (ATCC VR-260)	A B	≥5.0 log₁₀ ≥5.0 log₁₀	10 minutes
*Herpes Simplex Type 2 (ATCC VR-734)	A B	≥6.0 log₁₀ ≥6.0 log₁₀	10 minutes
*Human Coronavirus (ATTC VR-740, strain 229E)	A B	≥3.0 log₁₀ ≥3.0 log₁₀	10 minutes
*Human Immunodeficiency Virus, HTLV-IIIRF, strain of HIV-1 (associated with AIDS)	A B	≥3.5 log₁₀ ≥3.5 log₁₀	10 minutes
*Human Immunodeficiency Virus type 2 (HIV-2), strain CBL-20	A B	≥3.25 log₁₀ ≥3.25 log₁₀	10 minutes
*Influenza A2, strain Hong Kong (ATCC VR-544)	A B	≥4.25 log₁₀ ≥4.25 log₁₀	10 minutes
*Paramyxovirus (Mumps) (ATCC VR-1438)	A B	≥3.0 log₁₀ ≥3.0 log₁₀	10 minutes
*Porcine Respiratory & Reproductive Syndrome Virus (PRRSV), strain NVSL	A B	≥5.0 log₁₀ ≥5.0 log₁₀	10 minutes
*Pseudorabies, strain Aujeszky (ATCC VR-135)	A B	≥5.25 log₁₀ ≥5.25 log₁₀	10 minutes
*Rabies Virus (attenuated CDC ERA strain)	A B	3.0 log₁₀ 3.0 log₁₀	10 minutes
*Rotavirus, strain SA-11 (ATCC VR-899)	A B	4.5 log₁₀ 4.5 log₁₀	10 minutes
*SARS Associated Coronavirus (ZeptoMetrix)	A B	3.03 log₁₀ 3.03 log₁₀	10 minutes
*Vaccinia, strain WR (ATCC VR-119)	A B	≥5.5 log₁₀ ≥5.5 log₁₀	10 minutes

Conclusions: Under the conditions of this investigation, Calla^® 1452 demonstrated virucidal activity against SARS-Related Coronavirus 2 (SARS-CoV-2), Avian Influenza A Virus (H3N2), Avian Influenza Virus, Type A (H9N2), Bovine Rhinotracheitis, Bovine Viral Diarrhea Virus (BVDV), Canine Distemper Virus, Feline Leukemia (VR-717), Feline Picornavirus, Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Herpes Simplex Type 1, Herpes Simplex Type 2, Human Coronavirus, Human Immunodeficiency Virus (HIV-1), Human Immunodeficiency Virus Type 2 (HIV-2), Influenza A2, Paramyxovirus (Mumps), Porcine Respiratory & Reproductive Syndrome Virus (PRRSV), Pseudorabies, Rabies Virus, Rotavirus, SARS Associated Coronavirus, and Vaccinia, according to criteria established by the U.S. Environmental Protection Agency for registration and labeling of a disinfectant product as a virucide.

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